P-84: Protective Effect of Black Grapes Seed Hydroalcholic Extract Against Fluoxetine-Induced Embryo Toxicity in Male Mice

Background: Fluoxetine (FLX) is a selective serotonin reuptake inhibitors antidepressant. FLX affects chemicals in the brain that may become unbalanced and cause depression, panic, anxiety, or obsessive-compulsive symptoms. Black Grape seed extract (BGSE) antioxidants neutralize free radicals and oxidative stress. Therefore this study was designed to investigate Protective effect of RGSE hydroalcholic extract against fluoxetine -induced damage on embryo development. Materials and Methods: To follow up present study 24 mature male mice were used. The animals divided into four groups as, control, FLX (20 mg/kg), FLX (20 mg/kg) + BGSE (100 mg/kg) and BGSE (100 mg/kg) orally for 42 days. The caudae epididymides spermatozoa were obtained on day 42 in all groups. Superovulation was induced in 7-8 weeks-old female mice by intraperitoneal injection of 10 IU PMSG followed by intraperitoneal injection of 10 IU HCG 48 hours later. 1×106 sperm /ml HTF+ 4mg/kg BSA medium add to fertilization medium containing 10 to 15 oocytes. Fertilized oocytes or zygotes were evaluated. 24 hours after the zygotes culture, the two cell embryos rate was assessed and in vitro embryonic development was evaluated. Results: The results showed that in FLX group percentage of zygote, Blactocyst as well as hatching embryos significantly (p<0.05) reduced in comparison with the control, BGSE and FLX+ BGSE groups. There was no significant value between groups in two cell embryo percentage. Conclusion: BGSE extract caused partially improvement in all above mentioned embryos parameters. Therefore, grape seed extract can have positive effects on male fertility.